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Preclinical Scientific Evaluations of OsteoSponge

Evidence for Osteoinductive and Osteoconductive Properties

In Vivo Studies (Heterotopic -Bone Growth Model)

Bacterin has performed several studies using a heterotopic bone growth model in athymic mice to test the osteoinductive properties of OsteoSponge™,  OsteoSponge™ is prepared using methods that preserve native growth factors contained within the collagen matrix, Neovascularization, osteoid formation and new bone growth has been consistently observed in subcutaneously-implanted OsteoSponge™

The results suggest that in addition to providing an excellent natural scaffold for cellular ingrowth and proliferation, OsteoSponge™ also contains growth factors.  Growth factors signal infiltrating host cells to initiate the formation of new blood vessels and to differentiate into osteoclasts and osteoblasts.  This combination of signaling and structure ultimately leads to the formation of new bone.


Endogenous Native Growth Factors in OsteoSponge™

Characterization of the native growth factor content of OsteoSponge™ aids in understanding its osteoinductive activity.  To evaluate the levels of endogenous growth factors in OsteoSponge™, Enzyme-Linked Immunosorbent Assays (ELISAS) were used to measure the levels of native growth factors, including human bone morphogenic proteins (hBMPs), in extracts of OsteoSponge™ blocks processed from a 27 year old male donor.  Shown in Table 1 are levels of hBMP-2, hBMP-4 and hBMP-7 and insulin-like growth factor-1 (IGF-1)


Growth Factor Level Detected (pg/g of bone)
BMP-2 8,423 ± 248
BMP-4 1,112 ± 19
BMP-7 50,581 ± 1,876
IGF-1 713 ± 173

 These results illustrate that OsteoSponge™ provides a host of signaling molecules that are involved in the remodeling and creation of new bone.  Therefore, OsteoSponge™ supports the natural progression of events from bone induction to bone formation.


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Last modified: 08/16/10